Fig. 8

Co-localization of FMR antibodies. a Immunofluorescence in control and FXTAS brain tissue co-stained with UB and p62 (left panels) and UB and UBQLN2 (right panels). Nuclei were stained with DAPI. Scale bar = 10 μm. b Immunofluorescence in control and FXTAS brain tissue co-stained with UB and either CTF1, NTF1, FMRpolyA, ASFMRpolyP or ASFMRpolyA. Nuclei were stained with DAPI. Scale bar = 10 μm. c Graph comparing the percent of inclusions that were either UB positive and co-stain negative, UB negative and co-stain positive, or both UB positive and co-stain positive. Chi-squared test with Bonferroni Correction for multiple comparisons. N > 50 inclusions/comparison. **** p < 0.0001. d Immunofluorescence in control and FXTAS brain tissue co-stained with p62 and CTF1 (left panels) and p62 and NTF1 (middle panels). Nuclei were stained with DAPI. Scale bar = 10 μm. Graph comparing the percent of inclusions that were either p62 positive and CTF1/NTF1 negative, UB negative and CTF1/NTF1 positive, or UB positive and CTF1/NTF1 positive (right). Chi-squared test with Bonferroni correction for multiple comparisons. N > 50cells/comparison. **** p < 0.0001. e Immunofluorescence in control and FXTAS brain tissue co-stained with NTF1 and UBQLN2. Nuclei were stained with DAPI. Scale bar = 10 μm. Graph comparing the percent of inclusions positive for NTF1 and UBQLN2 (right). Chi-squared test with Bonferroni correction. **** p < 0.0001