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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: Neuropathology of RAN translation proteins in fragile X-associated tremor/ataxia syndrome

Fig. 5

N-terminal targeted FMRpolyG antibody staining in FXTAS tissue. a Representative images from control (left) and FXTAS (right) hippocampus stained with NTF1 antibody. Nuclei stained with hematoxylin. Inset- 60x magnification. Scale bar = 20 μm. b Quantification of a represented as percent neurons with NTF1 positive aggregates in hippocampus. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. c Graph showing the percentage of cells with NTF1 positive aggregates that are neurons or glia in FXTAS hippocampus. d Graph comparing average staining intensity for NTF1 positive aggregates between control and FXTAS hippocampus. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. e Representative images from control (left) and FXTAS (right) cortex stained with NTF1 antibody. Nuclei stained with hematoxylin. Inset- 60x magnification. Scale bar = 20 μm. f Quantification of E represented as percent neurons with NTF1 positive aggregates in cortex. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. g Graph showing the percentage of cells with NTF1 positive aggregates that are neurons or glia in FXTAS cortex. h Graph comparing average staining intensity for NTF1 positive aggregates between control and FXTAS cortex. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. I Representative images showing UB & NTF1 staining of control, SCA3, C9ORF72/ALS-FTD and FXTAS brain. Nuclei stained with hematoxylin. Scale bars = 20 μm. j Quantification of i showing percent neurons with UB and NTF1 positive aggregates in control, FXTAS, C9ORF72/ALS-FTD and SCA3 tissues. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001

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