Fig. 1

Staining for pathological markers Ubiquitin, p62, and UBQLN2 in FXTAS brain. a Representative images from control (left) and FXTAS (right) hippocampus (upper panels) and cortex (lower panels) stained for ubiquitin. Nuclei stained with hematoxylin. Inset- 60x magnification. Scale bar = 20 μm. b Quantification of a represented as percent neurons with aggregates. Data from hippocampus (top) and cortex (bottom). Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. c Graph showing the percentage of cells with ubiquitin positive aggregates that are neurons and glia in hippocampus (top) and cortex (bottom) in FXTAS tissue. d Graph comparing the average staining intensity for ubiquitin in hippocampus (top) and cortex (bottom) between control and FXTAS tissue. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. e Representative images from control (left) and FXTAS (right) hippocampus (upper panels) and cortex (lower panels) stained for p62. Nuclei stained with hematoxylin. Inset- 60x magnification. Scale bar = 20 μm. f Quantification of e represented as percent neurons with aggregates. Data from hippocampus (top) and cortex (bottom). Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. g Graph showing the percentage of cells with p62 positive aggregates that are neurons and glia in hippocampus (top) and cortex (bottom) in FXTAS tissue. h Graph comparing average staining intensity for p62 in hippocampus (top) and cortex (bottom) between control and FXTAS tissue. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001. i Representative images from control (left) and FXTAS (right) hippocampus (upper panels) and cortex (lower panels) stained for UBQLN2. Nuclei stained with hematoxylin. Inset-60x. Scale bar = 20 μm. j Quantification of i represented as percent of neurons with aggregates. Data from hippocampus (top) and cortex (bottom). Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001, ns = not significant. k Graph showing the percentage of cells with UBQLN2 positive aggregates that are neurons or glia in hippocampus (top) and cortex (bottom) from FXTAS tissue. l Graph comparing average staining intensity for UBQLN2 in hippocampus (top) and cortex (bottom) between control and FXTAS tissue. Results expressed as means ± SEM; Mann-Whitney U-test **** p < 0.0001