Fig. 3

Early lesion phenotype spatial distributions and transition analyses. (a, b) Spatial separation of (a) myeloid cell and (b) astrocyte phenotypes into NAWM, rim and center lesion zones. (c) T cells are primarily located in the lesion center. (d) PHATE mapping of myeloid cells, indicating that the G/WM outer rim (m1) and lesion center phenotypes (m2) are on a transition continuum (white arrow). (e) Pseudotime analysis of myeloid cells shows that phenotypes transition along independent trajectories. Phenotype color schemes on the PHATE and Pseudotime plots reflect the color palettes specific to each analysis. Scale bar for a-c = 200 μm. G/WMoR = gray and white matter outer rim; WMoR = white matter outer rim; iR = inner rim; WM R/C = white matter rim/center; C = center; NAWM = normal-appearing white matter