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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: The landscape of myeloid and astrocyte phenotypes in acute multiple sclerosis lesions

Fig. 1

Single-cell segmentation. (a-c) Segmentation of myeloid cells (CD68, magenta), astrocytes (S100B, green) and T cells (CD3, cyan) performed on CellProfiler. (b) shows cells in (a) at higher magnification. (c) shows examples of S100B+ astrocyte processes (white arrows) that closely neighbor CD68+ cells, which resulted in the co-segmentation of these markers in a small fraction of cells. (d) Gating of CD68+ and S100B+ populations on a flow cytometry plot as a quality control for segmentation (late lesion). The overlap population consists of cells with co-segmented CD68 and S100B like those in (c). Scale bar in (a) = 30 μm; (b) = 15 μm; (c) = 30 μm, inset = 10 μm. Z-score normalized expression intensities are shown (d)

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