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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: TERT expression is susceptible to BRAF and ETS-factor inhibition in BRAFV600E/TERT promoter double-mutated glioma

Fig. 5

Activation of the mutant TERT promoter by ETS1. a An siRNA approach was applied to ETS1 knock-down in cell models with different BRAF and TERT promoter status as indicated. TERT mRNA expression was detected using qRT-PCR. Significance levels were evaluated by unpaired students’ t-tests (mean +/− SEM). b Binding of ETS1 and GABPA to the different TERT promoter variants and presence of the activating histone mark H3K27-Ac was analyzed by ChIP-qRT-PCR. IgG served as negative control. Values are given as % Input, depicted mean +/− SD from duplicates. c Cytotoxicity assay were performed in cell models of different BRAF and TERT promoter background as depicted. Half-maximal inhibitory concentration (IC50) after YK-4-279 treatment for 72 h was calculated. Tukey’s multi-comparison one-way ANOVA was applied (mean +/− SD). d TERT and ETS1 mRNA expression levels were analyzed by qRT-PCR after 16 h treatment with the indicated concentrations of YK-4-279. Ordinary one-way ANOVAs for every cell line were calculated (Dunnet correction, 0.05 (95% confidence interval) as controls vs. treatments (mean +/− SEM). *p < 0.05, **p < 0.01, ***p < 0.001, wt = wild-type, mut = mutated, n.d. = not detected

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