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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: TERT expression is susceptible to BRAF and ETS-factor inhibition in BRAFV600E/TERT promoter double-mutated glioma

Fig. 3

Regulation of TERT expression and TERT promoter activity upon BRAF-inhibition. a TERT mRNA expression following dabrafenib treatment (1 μM, 16 h) of the indicated cell models is shown. Mean +/− SD; unpaired student’s t-tests (b) Luciferase reporter assays were performed in cell lines with different BRAF and TERT promoter status as indicated using wild-type or mutated (C228T) TERT promoter sequences. Cells were treated with 1 μM dabrafenib for 16 h. Results are given as ratio of firefly to renilla luciferase (internal control) and were normalized to a promoter-less construct (−Co, set to 1). Values are given as mean +/− SD from duplicates. One representative experiment out of three, delivering comparable results, is shown. Tukey’s multi-comparison one-way ANOVA was applied for statistical analysis. *p < 0.05, **p < 0.01, ***p < 0.001; wt = wild-type, mut = mutated, n.d. = not detected, dab = dabrafenib

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