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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: TERT expression is susceptible to BRAF and ETS-factor inhibition in BRAFV600E/TERT promoter double-mutated glioma

Fig. 2

Anti-proliferative effects and altered downstream-signaling upon BRAF-inhibition. a Clone formation assays of with different BRAF and TERT promoter status as indicated are shown. Cells were seeded at low density and treated with 1 μM dabrafenib for 7 days. The upper panel depicts one representative well per condition. The lower panel shows the quantitative results represented as mean +/− SD of the respective untreated control. ***p < 0.001 (unpaired student’s t-tests) (b) Western blot analyses of cell models with different BRAF and TERT promoter status are depicted. Cell models were treated with 1 μM dabrafenib for 6 h. Expression and phosphorylation of the indicated MAPK pathway mediators as well as cyclin D1 are shown. Fold values are given as normalized expression to β-actin followed by activated kinase/total kinase and are normalized to the respective control. wt = wild-type, mut = mutated, dabra = dabrafenib

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