Table 1 Primary antibodies used for immunofluorescence
Antigen | Host | Dil. | HIER | Catalog No. | Company |
|---|---|---|---|---|---|
Glial fibrillary acidic protein (GFAP) | Mouse | 1:500 | Yes | G-3893 | Sigma, St Louis, USA |
Allograft inflammatory factor 1 (IBA1) | Rabbit | 1:250 | Yes | 019–19741 | Wako, Neuss, Germany |
Neurofilament-M (NEFM) | Mouse | 1:500 | No | RMO-270 | Thermo Fisher Scientific, Waltham, USA |
CD3 | Rabbit | 1:100 | Yes | NB600–1441 | Novus Biologicals, Littleton, USA |
Albumin (ALB) | Rabbit | 1:500 | No | RARaAlb | Nordic Immunology, Tilburg, Netherlands |
Myelin basic protein (MBP) | Rabbit | 1:1000 | No | ab216668 | Abcam, Cambridge, UK |
Neuronal nuclei (NEUN) | Mouse | 1:250 | No | MAB377 | Merck Millipore, Billerica, USA |
Glutamine synthetase (GS) | Mouse | 1:250 | Yes | MAB302 | Merck Millipore, Billerica, USA |
Alzheimer precursor protein (APP) | Mouse | 1:100 | Yes | MAB348 | Merck Millipore, Billerica, USA |
Aquaporin-4 (AQP4) | Goat | 1:250 | No | sc-9888 | Santa Cruz Biotechnology, USA |
Transmembrane protein 119 (TMEM119) | Rabbit | 1:200 | Yes | G-9264 | Sigma, St Louis, USA |
CD68 | Mouse | 1:100 | Yes | MAB1435 | Merck Millipore, Billerica, USA |