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Table 1 Primary antibodies used for immunofluorescence

From: Retinal pathology in experimental optic neuritis is characterized by retrograde degeneration and gliosis

Antigen Host Dil. HIER Catalog No. Company
Glial fibrillary acidic protein (GFAP) Mouse 1:500 Yes G-3893 Sigma, St Louis, USA
Allograft inflammatory factor 1 (IBA1) Rabbit 1:250 Yes 019–19741 Wako, Neuss, Germany
Neurofilament-M (NEFM) Mouse 1:500 No RMO-270 Thermo Fisher Scientific, Waltham, USA
CD3 Rabbit 1:100 Yes NB600–1441 Novus Biologicals, Littleton, USA
Albumin (ALB) Rabbit 1:500 No RARaAlb Nordic Immunology, Tilburg, Netherlands
Myelin basic protein (MBP) Rabbit 1:1000 No ab216668 Abcam, Cambridge, UK
Neuronal nuclei (NEUN) Mouse 1:250 No MAB377 Merck Millipore, Billerica, USA
Glutamine synthetase (GS) Mouse 1:250 Yes MAB302 Merck Millipore, Billerica, USA
Alzheimer precursor protein (APP) Mouse 1:100 Yes MAB348 Merck Millipore, Billerica, USA
Aquaporin-4 (AQP4) Goat 1:250 No sc-9888 Santa Cruz Biotechnology, USA
Transmembrane protein 119 (TMEM119) Rabbit 1:200 Yes G-9264 Sigma, St Louis, USA
CD68 Mouse 1:100 Yes MAB1435 Merck Millipore, Billerica, USA
  1. HIER heat-induced epitope retrieval, Dil. Dilution