Fig. 3

Microgliosis presented early in EAE and persisted until the final observational time point. Increased microglial cell (IBA1) presence was observed as early as 7 days post immunisation (dpi) in both the a/b optic nerve and d retina (arrows), persisting until the final observational time point in EAE mice. b TMEM119 marked resident microglial cells observed at 7 dpi and 11 dpi in EAE mice, a while in the optic nerve, a significant increase in microglial cells at 11 dpi also coincided with cellular infiltration. The insets a/c display examples of microglial cells in controls (ramified: weak signal, long branched processes) and EAE mice (amoeboid: strong signal, round)