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Table 1 Primary antibodies used for immunoflurorescence staining

From: Glial pathology and retinal neurotoxicity in the anterior visual pathway in experimental autoimmune encephalomyelitis

Antibody

Source

Dilution

Mouse anti-NeuN

Millipore

1:100

Goat anti-Brn3a

Santa Cruz Biotech

1:100

Rabbit anti-RBPMS

GeneTex

1:100

anti-β-III tubulin

BD Pharmingen

1:100

anti-Synaptophysin

Millipore

1:100

Anti-PSD-95

ThermoFisher Scientific

1:200

Rabbit anti-IBA-1

Wako

1:500

Rabbit anti-GFAP

DAKO

1:500

Rat anti-C3

Hycult Biotech

1:200

Rat anti-CD4

Biolegend

1:100

Mouse anti-SMI31

Biolegend

1:250

Mouse anti-SMI32

Biolegend

1:250

Rabbit anti-MBP

Cell Signaling Technology

1:250

Mouse anti-GFAP

Cell Signaling Technology

1:250 ON,

1:500 Retina

Anti-iNOS

Santa Cruz Biotech

1:1500

Anti-PSMB8

Abcam

1:100

Rat anti-MAC2

Biolegend

1:100