Fig. 6

Astrocytes and microglia cells were activated in the optic nerve of EAE. a GFAP and IBA1 staining in the optic nerve of EAE mouse. b Quantification of GFAP staining in optic nerve. c Quantification of IBA1 staining in optic nerve. d IBA1 and iNOS double staining in the optic nerve of EAE. e Quantification of iNOS staining in optic nerve. Boxed regions from each image were magnified as shown in figures. White dashed lines delineate the ROI. Quantification is shown as mean ± SEM. Significance was determined by two-tailed, unpaired Student’s t-test with P < 0.05 consider as significant. * P ≤ 0.05, ** P ≤ 0.01, *** P ≤ 0.001. Scale bar =50 μm