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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: Glial pathology and retinal neurotoxicity in the anterior visual pathway in experimental autoimmune encephalomyelitis

Fig. 3

Glial cells were activated in the inner retina of EAE mouse, with increased complement component 3 (C3) activation in GFAP+ glia cells. a IBA1 staining in inner retina of EAE mice. In CFA control mice, IBA1+ microglia cells were located in the inner retina with slim processes, distributed in GCL layer, deep IPL layer and OPL layer. However, in EAE retinas, IBA+ cells had more round ameba-liked shape and less ramified morphology, indicating activation of microglial (a). b-c Quantification of IBA1 staining in EAE (n = 17) and CFA control mice (n = 13) in GCL and IPL layer of the inner retina. d GFAP staining in inner retina of EAE mice. In CFA control mice, GFAP+ cells were mostly located in the GCL and slightly in the OPL. However, in EAE mice retinas, GFAP+ glia cells had longer processes, which extended from the GCL into the deeper IPL (d). e-f Quantification of GFAP staining in the inner retina of EAE (n = 17) and CFA (n = 13) control mice in GCL and IPL layer (e) and IPL layer (f) only. g C3 and GFAP double staining in retina of EAE and CFA control mice. h Quantification of C3 staining in GCL layer of EAE (n = 17) and CFA (n = 13) control mice. In EAE mice, GFAP+ cells had higher C3 expression in the RGC layer compared with CFA control mice. Quantification is shown as mean ± SEM. Significance was determined by two-tailed, unpaired Student’s t-test with P < 0.05 considered as significant. ** P ≤ 0.01, *** P ≤ 0.001. N.S. = no significant difference. Scale bar =20 μm

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