Fig. 2

Neurite projection and synaptic density were lost in inner retina of EAE mice at PID42. a Schematics for mouse eyeballs verticle section and retinal region selection. b Neurite projections in inner retina was stained with β-III-tubulin (Tuj). c Quantification of Tuj positive neurites in the inner plexiform layer (IPL) of retina in EAE mice (n = 17) vs CFA control mice (n = 9). d Presynaptic marker, synaptophysin (SYP), and the postsynaptic marker, PSD95 staining in CFA and EAE mouse retina. e, f Quantification of PSD-95 (e) and SYP (f) staining intensity in the inner retina of EAE mouse (n = 15) and CFA control (n = 9). Quantification of Tuj, SYP and PSD95 is represented as mean ± SEM. Significance was determined by two-tailed, unpaired Student’s t-test with P < 0.05 considered signficant. ** P ≤ 0.01. N.S. = no significant difference. Scale bar =20 μm