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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: A soluble truncated tau species related to cognitive dysfunction and caspase-2 is elevated in the brain of Huntington’s disease patients

Fig. 2

Levels of soluble total tau proteins are lowered in HD patients of the large, but not the small, cohort. a, d, g Representative IP/WB showing that the tau-13-immunoprecipitated soluble total tau (T-tau) proteins (vertical bars) were detected by the biotin-conjugated tau-5 antibody in the prefrontal cortex (Brodmann’s area (BA) 8/9) of subjects from the NIH NeuroBioBank and the New York Brain Bank (a), in the caudate nucleus of subjects from the NIH NeuroBioBank (d), and in the prefrontal cortex (BA 8) of subjects from the HUB-ICO-IDIBELL Biobank, Spain (g). HD, Huntington’s disease patients; non-HD, individuals without Huntington’s disease. +, in vitro synthetic full-length tau protein (hash; 20 μL of sample used in IP, positive control). Sample IDs and disease diagnoses (Additional file 1: Table S1) were shown for IP reactions. Ab only, IP reaction containing only 10 μg of tau-13 (negative control). MsIgG/br. ex., IP reaction containing 10 μg of mouse IgG and 173 μg of soluble brain proteins (negative control). For the MsIgG/br. ex. lanes in figure a, brain extracts were a mixture of the nine samples studied in the same blot with each contributing 1/9th the amount (i.e., 19.2 μg); for the MsIgG/br. ex. lanes in figure d, 1/8th the amount (i.e., 21.6 μg). b, e, h Comparison of levels of T-tau proteins in the prefrontal cortex (BA 8/9) of HD patients and non-HD individuals from the NIH NeuroBioBank and the New York Brain Bank (b), in the caudate nucleus, from the NIH NeuroBioBank (e), and in the prefrontal cortex (BA 8), from the HUB-ICO-IDIBELL Biobank, Spain (h). c, f Comparison of levels of Δtau314 proteins, normalized to levels of T-tau proteins, in the prefrontal cortex (BA 8/9) of HD patients and non-HD individuals from the NIH NeuroBioBank and the New York Brain Bank (c), and in the caudate nucleus, from the NIH NeuroBioBank (f). The numbers of analyzed subjects are shown in parentheses. A.U. = arbitrary unit. The y-axes in figures e, f and h are in log scale. Mann-Whitney test was used for between-group comparison; medians (middle long bars), and 1st (lower short bars) and 3rd (upper short bars) quartiles are shown

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