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Table 1 Agents administered to the mouse. The table summarizes all agents injected including company, injection site, volume, concentration, vehicle, administration time, sacrifice time, and number of retinas used

From: Retinal ischemia induces α-SMA-mediated capillary pericyte contraction coincident with perivascular glycogen depletion

Agent Company Injection route Volume Concentration (Vehicle) Time of administration Time of sacrifice n of mice
Tissue plasminogen activator (tPA) Actilyse; INN alteplase, Boehringer Ingelheim, Germany Tail vein 50 μl/min (1 min) + 15 μl/min (30–60 min) 10 mg/kg 1 h after ischemia 30–60 min after tPA injection 17
Dextran (FITC–70S or/and TRITC–500S) Sigma Tail vein 0.2 ml 5 mg/ml (saline) 1 h before ischemia and 1 h after tPA injection 1 h after ischemia/recanalisation 34
Adenosine Molecular Probes Intravitreal 3 μl 1 mg/ml (water) Just before ischemia 1 h after ischemia 4
1 h after ischemia 2 h after ischemia 3
Amlodipine Pfizer Intravitreal 3 μl 1 mg/ml (water) 1 h before ischemia 1 h after ischemia 3
Carbenoxolone (CBX) Sigma Intravitreal 3 μl 30 μM (water) Just before ischemia 1 h after ischemia 4
Fluorescent phalloidin Biotium Intravitreal 3 μl 200 U/ml (water) 1–2 h before ischemia 1 h after ischemia 5
Propidium iodide (PI) Molecular Probes, Invitrogen Intravitreal 1 μl 1 mg/ml (water) 1 h after ischemia 4–6 h after ischemia 5
Tissue plasminogen activator (tPA) + Fluorescent phalloidin Actilyse; INN alteplase, Boehringer Ingelheim, Germany; Biotium Tail vein Intravitreal 50 μl/min (1 min) + 15 μl/min (30–60 min)
3 μl
10 mg/kg
200 U/ml (water)
1 h after ischemia 30 min after ischemia 1 h after tPA injection 6
Fluo-4 Molecular Probes, Invitrogen Intravitreal 3 μl 5 μM (0.005% pluronic acid, 0.00025% cremophor and 0.05% DMSO in artificial CSF) Just before ischemia 2 h after ischemia 18
Scrambled peptide Smart-Bioscience Intravitreal 2 μl 20 μM (nuclease free water) 1 h before ischemia 1 h after ischemia 6
Peptide Smart-Bioscience Intravitreal 2 μl 20 μM (nuclease free water) 1 h before ischemia 1 h after ischemia 6
1,4-Dideoxy-1,4-imino-D-arabinitol hydrochloride (DAB) Sigma-Aldrich Intravitreal 3 μl 2.5 M (saline) 2 h before ischemia 30 min after ischemia 9
Scrambled siRNA Ambion® LifeTech, Intravitreal 3 μl 0.5 mg/ml (10% Glucose, nuclease free wáter, in~vivo-jetPEI®, 10% Glucose) 48 h before ischemia 1 h after ischemia 3
α SMA siRNA Ambion® LifeTech, Intravitreal 3 μl 0.5 mg/ml (10% Glucose, nuclease free wáter, in~vivo-jetPEI®, 10% Glucose) 48 h before ischemia 1 h after ischemia 3