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Table 1 Agents administered to the mouse. The table summarizes all agents injected including company, injection site, volume, concentration, vehicle, administration time, sacrifice time, and number of retinas used

From: Retinal ischemia induces α-SMA-mediated capillary pericyte contraction coincident with perivascular glycogen depletion

Agent

Company

Injection route

Volume

Concentration (Vehicle)

Time of administration

Time of sacrifice

n of mice

Tissue plasminogen activator (tPA)

Actilyse; INN alteplase, Boehringer Ingelheim, Germany

Tail vein

50 μl/min (1 min) + 15 μl/min (30–60 min)

10 mg/kg

1 h after ischemia

30–60 min after tPA injection

17

Dextran (FITC–70S or/and TRITC–500S)

Sigma

Tail vein

0.2 ml

5 mg/ml (saline)

1 h before ischemia and 1 h after tPA injection

1 h after ischemia/recanalisation

34

Adenosine

Molecular Probes

Intravitreal

3 μl

1 mg/ml (water)

Just before ischemia

1 h after ischemia

4

1 h after ischemia

2 h after ischemia

3

Amlodipine

Pfizer

Intravitreal

3 μl

1 mg/ml (water)

1 h before ischemia

1 h after ischemia

3

Carbenoxolone (CBX)

Sigma

Intravitreal

3 μl

30 μM (water)

Just before ischemia

1 h after ischemia

4

Fluorescent phalloidin

Biotium

Intravitreal

3 μl

200 U/ml (water)

1–2 h before ischemia

1 h after ischemia

5

Propidium iodide (PI)

Molecular Probes, Invitrogen

Intravitreal

1 μl

1 mg/ml (water)

1 h after ischemia

4–6 h after ischemia

5

Tissue plasminogen activator (tPA) + Fluorescent phalloidin

Actilyse; INN alteplase, Boehringer Ingelheim, Germany; Biotium

Tail vein Intravitreal

50 μl/min (1 min) + 15 μl/min (30–60 min)

3 μl

10 mg/kg

200 U/ml (water)

1 h after ischemia 30 min after ischemia

1 h after tPA injection

6

Fluo-4

Molecular Probes, Invitrogen

Intravitreal

3 μl

5 μM (0.005% pluronic acid, 0.00025% cremophor and 0.05% DMSO in artificial CSF)

Just before ischemia

2 h after ischemia

18

Scrambled peptide

Smart-Bioscience

Intravitreal

2 μl

20 μM (nuclease free water)

1 h before ischemia

1 h after ischemia

6

Peptide

Smart-Bioscience

Intravitreal

2 μl

20 μM (nuclease free water)

1 h before ischemia

1 h after ischemia

6

1,4-Dideoxy-1,4-imino-D-arabinitol hydrochloride (DAB)

Sigma-Aldrich

Intravitreal

3 μl

2.5 M (saline)

2 h before ischemia

30 min after ischemia

9

Scrambled siRNA

Ambion® LifeTech,

Intravitreal

3 μl

0.5 mg/ml (10% Glucose, nuclease free wáter, in~vivo-jetPEI®, 10% Glucose)

48 h before ischemia

1 h after ischemia

3

α SMA siRNA

Ambion® LifeTech,

Intravitreal

3 μl

0.5 mg/ml (10% Glucose, nuclease free wáter, in~vivo-jetPEI®, 10% Glucose)

48 h before ischemia

1 h after ischemia

3