Fig. 4
From: Reduction of ephrin-A5 aggravates disease progression in amyotrophic lateral sclerosis
Electromyography decline, neuronal loss and neuromuscular junction denervation in SOD1G93A mice is not affected in SOD1G93A mice with reduced efnA5 levels. a Compound muscle action potential amplitude (expressed in mV) was measured in SOD1G93A EfnA5+/+ and SOD1G93A EfnA5+/− mice from the age of 60 days (d) onwards. Data is represented as as mean ± SEM (N = 7–8). b The number of motor neurons of different soma area was counted in the ventral horn of the lumbar spinal cord. Data is represented as mean ± SEM (N = 6–7) and it was analysed with a two-way ANOVA. Representative image shows Neurotrace staining of a spinal cord slice. Scale bar = 50 μm. c and d Expression levels of c Rbfox3 and d Chat were unaltered after efnA5 knockdown in mice at 130 days of age. Data represents mean ± SEM (N = 6–7), and different conditions were compared with a one-way ANOVA with Tukey’s multiple comparisons test: * P < 0.05; ** P < 0.01; *** P < 0.001 as compared with SOD1WT mice. e Neuromuscular junctions (NMJs) innervation was scored in the gastrocnemius muscle. Data represents mean ± SEM (N = 5–6) and it was analysed with a two-tailed t-test. Representative images are shown of denervated and innervated NMJs in the gastrocnemius muscle that were stained with neurofilament-L (NfL) and α-bungarotoxin (BTX). Scale bar = 50 μm