Fig. 2

ACY-738 treatment restores histone acetylation. a Western blot of histone 3 acetylation levels in the spinal cord of P60 non-Tg controls, vehicle- and ACY-738-treated (100 mg/kg ACY-738 in chow) Tg FUS+/+ mice. Histone 4 levels were used as reference for equal loading. Hyperacetylation of histone 3 was used as a readout for class I HDAC inhibition. b Quantification of the ratio of acetylated histone 3 to total histone 4 levels and normalization to non-Tg controls. n = 4, One-way ANOVA with Tukey’s multiple comparisons test. *P < 0.05, **P < 0.01, ****P < 0.0001. Data are presented as means ± SEM