Fig. 1

Neuronal histone hypoacetylation is associated with progressive neurodegeneration in Tg FUS+/+ mice. a Number of α-motor neurons in the spinal cord of P28 and P60 Tg FUS+/+ mice and non-Tg controls. n = 4–6, Student’s t-test. b Percentage of innervated neuromuscular junctions in the gastrocnemius muscle of P28 and P60 Tg FUS+/+ mice and non-Tg controls. n = 3–6, Student’s t-test. c Muscle fiber area distribution profile of the gastrocnemius muscle of P28 and P60 Tg FUS+/+ mice and non-Tg controls. n = 3, Two-way ANOVA with Tukey’s multiple comparisons test. d Compound muscle action potential amplitudes measured at the gastrocnemius muscle of P28 and P60 Tg FUS+/+ mice and non-Tg controls. n = 3–4, Student’s t-test. e Western blot of histone 3 acetylation levels in the spinal cord of P25 and P60 Tg FUS+/+ mice and non-Tg controls. Total histone 4 levels were used as reference for equal loading. f Quantification of the ratio of acetylated histone 3 to total histone 4 levels and normalization to non-Tg controls. n = 3, Student’s t-test. g In situ nuclear HDAC activity measurement on spinal cord homogenates of P60 Tg FUS+/+ mice and non-Tg controls. n = 3, Student’s t-test. h Quantitative PCR analysis of mRNA expression levels of individual class I HDACs in the spinal cord of P60 Tg FUS+/+ mice and non-Tg controls, with Ap3b1 and Mon2 as reference genes and normalization to non-Tg controls. Fold change compared to non-Tg controls (FC). n = 6, Student’s t-test with Holm-Sidak method to correct for multiple testing. i Western blot of Hdac1, Hdac2 and Hdac3 in the spinal cord of P60 Tg FUS+/+ mice and non-Tg controls. Calnexin levels were used as reference for equal loading. j Quantification of the ratio of Hdac1, Hdac2 and Hdac3 to calnexin and normalization to non-Tg controls. *P < 0.05, ***P < 0.001, ****P < 0.0001. Data are presented as means ± SEM