Alzheimer’s disease neuropathological change and loss of matrix/neuropil in patients with idiopathic Normal Pressure Hydrocephalus, a model of Alzheimer’s disease

Table 1 Immunohistochemical stains

Antibody	Clone	Company/Code	Dilution	Pretreatment
Amyloid β (Aβ)	6F/3D	Dako-Agilent/M0872	1:50	98–100% FA
Embryonic lethal abnormal visual system proteins (nELAV) 3 and 4 human homolog HuC/HuD (HuC/HuD)	16A11	ThermoFisher Scientific/A-21271	1:2000	ac, CB
NEUronal Nuclei (NeuN)	A60	Milipore/MAB377	1:2000	CB
Hyperphosphorylated (Ser202/Thr205) τ (TAU8)	PHF-TAU-AT8	Fisher Sientific-Invitrogen/MN1020	1:1000
Synaptophysin (SYP)	SY38	Dako-Cytomation/M0776	1:50	CB

Dako Autostainer Plus (Dako Cytomation) was used for Aβ, Tau8 and SYP, while the HuC/HuD and NeuN were carried out manually, incubation at room temperature for 1 h. Autoclave (ac), formic acid (FA), citrate buffer pH 6.0 (CB)

ISSN: 2051-5960