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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Neutrophil extracellular trap induced by HMGB1 exacerbates damages in the ischemic brain

Fig. 4

Suppression of CitH3 induction in brain parenchyma and in the peripheral blood after MCAO by anti-HMGB1 antibody or HMGB1 A box. a Anti-HMGB1 antibody (200 μg/kg), HMGB1 A box (50 μg/kg), or IgG (200 μg/kg) were administered intravenously after 3 h of MCAO. b-c Levels of CitH3 in the cortices of ischemic hemispheres after 12 h of MCAO (b) and in neutrophils isolated after 12 h of MCAO (c) were examined by immunoblotting. GAPDH was used as a loading control. d-e Coronal brain sections (d) and blood neutrophils (e) were prepared after 12 h of MCAO and triple immunofluorescent staining was conducted using anti-CitH3 antibody, anti-Ly6g antibody, plus DAPI. Scale bars in D represent 100 μm and those in E represent 10 μm. f-g Coronal brain sections were prepared after 24 h of MCAO and mean infarct volumes were determined by TTC staining (f) and are presented as means±SEMs (g). pMCAO, PBS-treated pMCAO control (n = 6); anti-HMGB1, anti-HMGB1-treated pMCAO group (n = 7); HMGB1 A box, HMGB1 A box-treated pMCAO group (n = 7)

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