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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Sporadic Creutzfeldt-Jakob disease prion infection of human cerebral organoids

Fig. 6

Astrocyte changes during infection. a Whole organoid images at 96 dpi; left panels per condition show GFAP staining (pink) and right panels showing positive pixel counts (PPC), blue signifies low and yellow-orange denotes high pixel staining intensity. Scale bars = 500 μm. b GFAP positive pixel counts of the 96 dpi whole organoid images. Each data point represents an individual organoid with the mean indicated as a line (Kruskal-Wallis, p = 0.196). c Heatmap and dendrogram cluster analysis of cytokine detection in culture medium at 14, 35, 68, 95, 127, 159, 169 dpi. Black triangles showing increasing time from inoculation. Data points are generated using media pools from 6 individual organoids. Grey boxes indicate cytokine concentrations below the limits of detection. d Concentrations of Chitinase 3-like 1 in culture medium over time. # readings exceeded the accurate range of detection for the assay

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