Fig. 6
DLK inhibition has neuroprotective potential in cultures deprived from antioxidants. a Primary cultures deprived from antioxidants (-AO), display impaired neuronal network connectivity at DIV 14 (Morph.: nbio = 2 x ntech = 6 - Func.: nbio = 2 x ntech = 9); b Normalized connectivity scores of cultures deprived of antioxidants show that treatment from 0 DIV onwards with DLK inhibitors (GNE3511 and GNE8505) could prevent connectivity loss at 14 DIV (excl. Highest concentration) (Morph.: nbio = 2 x ntech = 6 - Func.: nbio = 2 x ntech = 9); c Representative images of control cultures, -AO cultures and -AO cultures treated with 1 μM GNE8505 (I, II and III in panel b); d Bar plots showing z-scores normalized to -AO cultures of dendrite density, synapse density and neuronal count (nbio = 2 x ntech = 6), as well as the absolute number of correlated calcium bursts (nbio = 2 x ntech = 9). Significant differences compared to control cultures or cultures deprived from antioxidants are indicated (p < 0.05, pairwise Wilcoxon test with Bonferroni correction)