Fig. 4
Focused compound assay identifies DLK as positive modulator of neuronal connectivity. a Connectivity score map after normalization to culture age-matched controls. Rapamycin is a strong negative modulator of neuronal connectivity and GNE3511 exerts a lasting positive effect on neuronal connectivity. (Morph.: nbio = 3 x ntech = 5 - Func.: nbio = 3 x ntech = 6, except for GNE3511: Morph.: nbio = 2 x ntech = 6 - Func.: nbio = 2 x ntech = 9); b Example images of control cultures and cultures treated with 0.1 μM rapamycin or GNE3511 (14 DIV) (I, II and III in panel a); c Z-scores showing the effect of 0.1 μM rapamycin or GNE3511 on dendrite density, synapse density, neuronal count (rapamycin: nbio = 3 x ntech = 5, GNE3511: nbio = 2 x ntech = 6) and correlation of calcium bursts (rapamycin: nbio = 3 x ntech = 6, GNE3511: nbio = 2 x ntech = 9)