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Fig. 2 | Acta Neuropathologica Communications

Fig. 2

From: High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity

Fig. 2

Culture age correlates with morpho functional changes. a Representative images of cortical cultures after fixation and immunocytochemistry at 6, 12, 18, 24, 30, 36, 42 and 48 DIV; b Dendrite network density and nodes increased gradually until 36 DIV, after which they decreased. The deterioration of the dendrite network was less pronounced when normalized to the number of neurons, which decreased over time. The presynaptic density and synapse density increased during the first 18 DIV. The density of postsynaptic spots increased gradually. The ratio between neuronal and non-neuronal nuclei remained more or less the same until 36 DIV (nbio = 2 x ntech = 6); c Representative immunocytochemistry images and Ca2+- traces (of 10 neurons) for a more resolved experiment (3, 7, 10, 14, 18 DIV) of primary hippocampal and cortical cultures; d Similar trends are observed in selected morphological descriptors for hippocampal and cortical cultures (nbio = 3 x ntech = 6). Synchronous activity increased from DIV 10 onwards (nbio = 3 x ntech = 6)

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