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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: High-throughput microscopy exposes a pharmacological window in which dual leucine zipper kinase inhibition preserves neuronal network connectivity

Fig. 1

General workflow. Workflow of the microscopy-based pipeline. Hippocampi and/or cortices from WT E18 C57Bl6 mouse embryos were dissected, after which cell suspensions were created and seeded in 96-well plates. Cultures that would be used in the functional assay were transfected with an AAVDJ-hSyn1-GCaMP6f-nls-dTomato vector on 0 DIV. At 3 DIV, cultures were treated with arabinosylcytosine (AraC) to suppress the excessive growth of non-neuronal cells. Cultures were treated every three or four days. At fixed time points, cultures were either fixed and subjected to immunostaining, or used for live cell imaging to assess the morphological and functional characteristics, respectively

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Acta Neuropathologica Communications

ISSN: 2051-5960

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