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Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: In vivo real-time dynamics of ATP and ROS production in axonal mitochondria show decoupling in mouse models of peripheral neuropathies

Fig. 5

ATP and H2O2 levels are different along axons. a Two-photons imaging of CFP (blue) and Venus (yellow) fluorescence of mito-ATeam was combined with CARS imaging of the myelin sheath (red). CARS imaging gap shows a node of Ranvier (arrowhead). Using the combined images, mitochondria located in the node of Ranvier and mitochondria located in internodes can be identified (right panel). Scale bar = 10 μm b Graph showing mito-ATeam fluorescence ratio in axonal mitochondria located in nodes of Ranvier (N) or internodes (IN) of wild-type (WT) or MFN2R94Q mice (MFN2 mutant). In wild-type mice, ATP levels are higher in mitochondria in nodes of Ranvier than in internodes (p = 0.035; n = 14 axons in 7 mice). In MFN2R94Q mice, ATP levels in node of Ranvier mitochondria are equal to internode mitochondria (n = 7 nodes in 3 mice). c Graph showing mito-roGFP-Orp1 fluorescence ratio in axonal mitochondria located in nodes of Ranvier or internodes of wild-type or mutant MFN2R94Q mice. In both wild-type mice and mutant MFN2R94Q mice, H2O2 levels are higher in mitochondria in nodes of Ranvier than in internodes (WT: p = 0.009; n = 8 axons in 3 mice. MFN2R94Q: p = 0.019; n = 9 axons in 3 mice). Error bars show SEM. Statistical tests are paired two-sided T-tests

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