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Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: In vivo real-time dynamics of ATP and ROS production in axonal mitochondria show decoupling in mouse models of peripheral neuropathies

Fig. 4

ATP and H2O2 levels of MFN2R94Q mice. a Graph showing mito-ATeam fluorescence ratio in resting axonal mitochondria of wild-type or mutant MFN2R94Q mice (Student two-tailed T-test p = 0.809; n = 18 axons, 7 mice per group). b Graph showing mito-ATeam fluorescence ratio normalized on pre-stimulation values (R/R0) following two successive nerve stimulation periods (black bars at the top). The first period of stimulation resulted in a slight, non-significant increase; the second stimulation period did not generate any change (n = 14 axons in 5 mice). c Graphs showing mito-ATeam R/R0 in wild-type (Fig. 3a) and mutant MFN2R94Q mice (b) were overlaid to show the relative dynamics of ATP levels in both genotypes after nerve stimulations (Two way ANOVA, p = 0.011 at t = 45, p = 4.8E-5 at t = 50, p = 0.02 at t = 55, F-value = 19,27; Df = 1). d Graph showing mito-roGFP-Orp1 fluorescence ratio in resting axonal mitochondria of wild-type or mutant MFN2R94Q mice (Student two-tailed T-test p = 0.337; n = 16 axons, 8 mice per group). e Graph showing mito-roGFP-Orp1 fluorescence ratio normalized on pre-stimulation values (R/R0) following two successive nerve stimulation periods (black bars at the top). Both stimulations result in a significant increase (p = 0.013 at t = 15, p = 0.020 at t = 20, p = 0.044 at t = 25 and p = 0.038 at t = 45; F-value = 3.095; Df = 14; n = 11 axons in 4 mice). f Graphs showing mito-roGFP-Orp1 R/R0 in wild-type (Fig. 3b) and mutant MFN2R94Q mice (e) were overlaid to show the relative dynamics of H2O2 levels in both genotypes after nerve stimulations. A significant higher H2O2 level is detected in wild-type first (Two way ANOVA, p = 0.007 at t = 5, F-value = 5484; Df = 1), then a higher H2O2 level is detected in mutant MFN2R94Q (p = 0.014 at t = 15, p = 0.047 at t = 40). Error bars show SEM. # = p-value< 0.05. ## = p-value< 0.01. ### = p-value< 0.001

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