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Fig. 3 | Acta Neuropathologica Communications

Fig. 3

From: In vivo real-time dynamics of ATP and ROS production in axonal mitochondria show decoupling in mouse models of peripheral neuropathies

Fig. 3

Effect of burst nerve stimulation on mitochondrial ATP and H2O2 levels. a Upper panels: Nerve stimulation induced changes in the fluorescence signal of both the CFP and Venus subunit of mito-ATeam as illustrated by the Venus/CFP overlay pictures. Lower panel: graph showing mito-ATeam fluorescence ratio normalized to pre-stimulation values (R/R0) following two successive nerve stimulation period (black bars at the top). The first stimulation results in a slight, non-significant increase; a significant increase is measured after a second stimulation (p = 0.0171 at t = 45, p = 0.0001 at t = 50, p = 0.049 at t = 55; F-value = 2.872; Df = 15; n = 9 axons in 3 mice). b Upper panels: Nerve stimulation induced changes in the fluorescence signal of both the oxidized and reduced forms of GFP in mito-roGFP-Orp1 as illustrated by the overlay pictures. Lower panel: graph showing mito-roGFP-Orp1 fluorescence ratio normalized on pre-stimulation values (R/R0) following two successive nerve stimulation (black bars at the top). Both stimulations result in a significant increase 5 min after the stimulation (p = 0.007; p = 0.04; F-value = 2.804; Df = 14; n = 14 axons in 7 mice). c Graphs described in a and b were overlaid to show the relative dynamics of ATP and ROS levels after nerve stimulations. Scale bars = 5 μm. Error bars show SEM. Statistical tests are one-way ANOVA

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