Fig. 5
Characterisation of the inflammation in c-MYC+ CPT and c-MYCOver CPT. a An increased T-lymphocytic infiltrate on CD3 immunohistochemistry in c-MYC+ tumours in relation to c-MYC- is noted (quantification showed on bar graph on the right; Mean ± SEM; n = 4 in each cohort; * P < 0.05). b Subtyping of T-lymphocytes showed predominant increase in CD4 subtype population (quantification showed on bar graph on the right; Mean ± SEM; n = 11 for c-MYC+ and n = 10 for c-MYC-; * P < 0.05). c Pronounced macrophage infiltration was also observed among c-MYC+ on CD68 immunohistochemistry (quantification showed on bar graph on the right; Mean ± SEM; n = 4 in each cohort; * P < 0.05). d, e Comparative analysis of the CPT (CPP) developing in the c-MYC overexpressing mouse model as compared to normal CP showed T-lymphocytic (d) and macrophagic (e) infiltration in the tumour parenchyma. Quantification is shown on bar graph on the right; d Mean ± SEM; n = 6 in CPT and n = 7 in control; *** P < 0.001 and e Mean ± SEM; n = 7 in CPT and n = 3 in control; ** P < 0.01. Scale bar = 125 μm (a, b, c, d, e)