Fig. 7

Phosphorylation of ERK was increased by treatment with HGF in the LSC and motor cortical cultures. a-c Motor cortices from non-TG or TG mice at P3 were collected. After dissociation, cells were seeded on six-well plates with 3.2 × 10⁶ cells/well. a Cells were treated with 100 ng/ml of rHGF, and 5 days later, total proteins were isolated, followed by Western blot analysis. b-c Cells were treated with 1 μM of PHA665752 or 10 μM of U0126. After 30 min, cells were treated with 100 ng/ml of rHGF, and five days later, total proteins were isolated followed by Western blot analysis. DMSO was used as a negative control. d The axon length of CSMNs was measured and represented as a bar graph after co-treatment with rHGF and U0126. For statistical analysis, one-way ANOVA was performed, followed by Tukey’s post-hoc test. e-f non-TG or TG mice at P60 were intrathecally injected with rAAV1-C or rAAV1-HGF. The LSCs were collected, and total proteins were extracted at P100, followed by Western blot analysis (e). Relative levels of phosphorylated ERK were measured using Fiji software and represented as a bar graph (f). For bar graphs, values are represented as mean ± SEM. For statistical analysis, one-way ANOVA was performed, followed by Tukey’s post-hoc test. ^*p < 0.05, ^**p < 0.01, ^***p < 0.001, ^****p < 0.0001