Fig. 1
IT administration of rAAV1-HGF promoted functional recovery in the sciatic nerve crush mouse model. a 4.12 × 108 GC of rAAV1, 2, 5, or 6-HGF were intrathecally injected into C57BL/6 mice at postnatal day 60 (P60). The LSCs were collected and total proteins were isolated 4 weeks after injection, followed by ELISA specific to human HGF (hHGF). For statistical analysis, one-way ANOVA was performed, followed by Tukey’s post-hoc test. ****p < 0.0001 for rAAV1 vs. other serotypes. b 1.4 × 109 GC of four serotypes were intrathecally injected into 2-month-old C57BL/6 mice. The LSCs were collected 4 weeks after injection. Tissues were fixed, followed by IHC assay using an antibody specific to GFP (green). The boundary between white and grey matter is distinguished by dotted lines, and white matter is indicated by white arrows. c 5 × 109 GC of rAAV1-HGF were intrathecally injected into 2-month-old C57BL/6 mice. The LSCs were collected 1, 2, 4, 8, 12, and 16 weeks after IT injection and subjected to ELISA for hHGF. For statistical analysis, two-way ANOVA was performed, followed by Sidak’s post-hoc test. The p-value between the two groups was 0.0002. d C57BL/6 mice at P60 were intrathecally injected with 5 × 109 GC of rAAV1-C or rAAV1-HGF. One week later, LSCs were collected and subjected to IHC assay. Antibodies specific to ChAT (green) and NeuN (red) were used to label SMNs, together with those for p-Met (magenta). In low magnification panels, the boundary between white and grey matter is distinguished by dotted lines. In merge panels, p-Met-expressing SMNs are indicated by white arrows. e The proportion of SMNs expressing p-Met per total SMNs was measured and represented as a bar graph. For statistical analysis, Student’s t-test was performed. ****p < 0.0001. In bar graphs, values are represented as mean ± SEM. Scale bar: b = 100 μm, d = 100 μm for low magnification panels and 20 μm for the others