Skip to main content
Fig. 4 | Acta Neuropathologica Communications

Fig. 4

From: Complement 3+-astrocytes are highly abundant in prion diseases, but their abolishment led to an accelerated disease course and early dysregulation of microglia

Fig. 4

A1-astrocyte markers are significantly altered in TKO mice upon prion infection a qPCR expression analysis of microglia and astrocyte disease markers in thalamus tissue from terminally sick mice and age matched control confirmed the knockout of TNFα in TKO mice, but significant upregulation in diseased WT-mice (p = 0.0004). A1-astrocyte markers are significantly less upregulated in diseased TKO-mice (C3 p = 0.0006; GBP2 p = 0.0001). In contrast, microglia disease marker Clec7a was upregulated in both infected groups (p = 0.0023) n = 3 independent mice/group. b Representative staining of GFAP (red), C3 (green) and DAPI (blue) in Thalamus tissue at clinical prion disease. c Quantification of positive staining area (μm2 × 1000) showed significant upregulation of GFAP in both, prion infected WT- and TKO-mice (p = 0.0003), while C3 is significantly upregulated in WT-mice only (p = 0.0005); n = 3 independent from Fig. 4a animals/group

Back to article page