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Table 4 Summary of investigations utilizing MSA derived brain lysate for in vivo αS pathology induction

From: Comparative analyses of the in vivo induction and transmission of α-synuclein pathology in transgenic mice by MSA brain lysate and recombinant α-synuclein fibrils

Mouse Model Inoculum Source αS Preparation Site of Inoculation Pathology Induction References
nTg Putamen sarkosyl insoluble intracerebral + Tarutani A et al. [43]
M83+/− Basal ganglia whole lysate intracerebral + M83 type Watts JC, et al. [49]
nTg Basal ganglia whole lysate intracerebral Prusiner SB, et al. [30]
Human WT αS/KOa Basal ganglia whole lysate intracerebral Prusiner SB, et al. [30]
M83+/− Basal ganglia whole lysate intracerebral + M83 type Prusiner SB, et al. [30]
M83+/− Substantia nigra whole lysate intracerebral + M83 type Woerman A, et al. [56]
M83+/− Substantia nigra sarkosyl insoluble/phosphotungstic precipitation intracerebral + M83 type Woerman A, et al. [56]
M83+/− Substantia nigra or basal ganglia whole lysate intraperitoneal + M83 type Woerman A, et al. [54]
M83+/− Substantia nigra or basal ganglia whole lysate intramuscular + M83 type Woerman A, et al. [54]
M83+/− Substantia nigra or basal ganglia whole lysate tongue + M83 type Woerman A, et al. [54]
M83+/+ Substantia nigra whole lysate intracerebral + M83 type Sargent D, et al. [41]
KOM2 Unknown brain region sarkosyl insoluble intracerebral + Peng C, et al. [28]
nTg Unknown brain region sarkosyl insoluble intracerebral + Peng C, et al. [28]
M83+/− Substantia nigra or basal ganglia whole lysate intracerebral + M83 type Woerman A, et al. [55]
Human WT αS/KOa Substantia nigra or basal ganglia whole lysate intracerebral Woerman A, et al. [55]
Human A30P αS/KOa Substantia nigra or basal ganglia whole lysate intracerebral Woerman A, et al. [55]
Human A53T αS/KOa Substantia nigra or basal ganglia whole lysate intracerebral + Woerman A, et al. [55]
  1. Indicated are the human brain regions that was used to generate the MSA lysates containing aggregated αS, the type of lysate preparation, the site of inoculation and the mouse lines used experimentally. Induction of CNS αS inclusion pathology is indicated by a +, with distribution typical of M83 mice indicated as + M83 type. KOM2 are transgenic mice expressing human WT αS specifically in oligodendrocytes [58] and crossed onto a murine αS null background [28]. aIndicates that these transgenic mice are homozygous for the respective form of human αS expressed from a P1 artificial chromosome and are on a murine αS null background [23]