Comparative analyses of the in vivo induction and transmission of α-synuclein pathology in transgenic mice by MSA brain lysate and recombinant α-synuclein fibrils

Dhillon, Jess-Karan S.; Trejo-Lopez, Jorge A.; Riffe, Cara; Levites, Yona; Sacino, Amanda N.; Borchelt, David R.; Yachnis, Anthony Y.; Giasson, Benoit I.

doi:10.1186/s40478-019-0733-3

Table 4 Summary of investigations utilizing MSA derived brain lysate for in vivo αS pathology induction

From: Comparative analyses of the in vivo induction and transmission of α-synuclein pathology in transgenic mice by MSA brain lysate and recombinant α-synuclein fibrils

Mouse Model	Inoculum Source	αS Preparation	Site of Inoculation	Pathology Induction	References
nTg	Putamen	sarkosyl insoluble	intracerebral	+	Tarutani A et al. [43]
M83^+/−	Basal ganglia	whole lysate	intracerebral	+ M83 type	Watts JC, et al. [49]
nTg	Basal ganglia	whole lysate	intracerebral	–	Prusiner SB, et al. [30]
Human WT αS/KO^a	Basal ganglia	whole lysate	intracerebral	–	Prusiner SB, et al. [30]
M83^+/−	Basal ganglia	whole lysate	intracerebral	+ M83 type	Prusiner SB, et al. [30]
M83^+/−	Substantia nigra	whole lysate	intracerebral	+ M83 type	Woerman A, et al. [56]
M83^+/−	Substantia nigra	sarkosyl insoluble/phosphotungstic precipitation	intracerebral	+ M83 type	Woerman A, et al. [56]
M83^+/−	Substantia nigra or basal ganglia	whole lysate	intraperitoneal	+ M83 type	Woerman A, et al. [54]
M83^+/−	Substantia nigra or basal ganglia	whole lysate	intramuscular	+ M83 type	Woerman A, et al. [54]
M83^+/−	Substantia nigra or basal ganglia	whole lysate	tongue	+ M83 type	Woerman A, et al. [54]
M83^+/+	Substantia nigra	whole lysate	intracerebral	+ M83 type	Sargent D, et al. [41]
KOM2	Unknown brain region	sarkosyl insoluble	intracerebral	+	Peng C, et al. [28]
nTg	Unknown brain region	sarkosyl insoluble	intracerebral	+	Peng C, et al. [28]
M83^+/−	Substantia nigra or basal ganglia	whole lysate	intracerebral	+ M83 type	Woerman A, et al. [55]
Human WT αS/KO^a	Substantia nigra or basal ganglia	whole lysate	intracerebral	–	Woerman A, et al. [55]
Human A30P αS/KO^a	Substantia nigra or basal ganglia	whole lysate	intracerebral	–	Woerman A, et al. [55]
Human A53T αS/KO^a	Substantia nigra or basal ganglia	whole lysate	intracerebral	+	Woerman A, et al. [55]

Indicated are the human brain regions that was used to generate the MSA lysates containing aggregated αS, the type of lysate preparation, the site of inoculation and the mouse lines used experimentally. Induction of CNS αS inclusion pathology is indicated by a +, with distribution typical of M83 mice indicated as + M83 type. KOM2 are transgenic mice expressing human WT αS specifically in oligodendrocytes [58] and crossed onto a murine αS null background [28]. ^aIndicates that these transgenic mice are homozygous for the respective form of human αS expressed from a P1 artificial chromosome and are on a murine αS null background [23]

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ISSN: 2051-5960

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