Fig. 5

Effects of APOE genotype and sex on AD-related pathology. a Representative images show ThioS-positive plaques (cyan) and Iba-1 immunoreactive microglia (white) in the HPC of EFAD mice. Scale bars = 15 μm. b Quantification of amyloid plaque load (% of total ROI area) in male (M) and female (F) EFAD mice with APOE3 (open bars) and APOE4 (filled bars) genotypes. c-d Quantification of microglia load (% of total ROI area) near (< 100 μm, C) and far (> 100 μm, D) from ThioS-positive plaques. e-f Quantification of activated microglial phenotype (mean soma area of Iba1-immunoreactive cells) near (< 100 μm, E) and far (> 100 μm, F) from ThioS-positive plaques. Numbers of mice analyzed per group are as follows: male E3FAD (n = 7), female E3FAD (n = 7), male E4FAD (n = 5), and female E4FAD (n = 6). * denotes p < 0.05, ** denotes p < 0.01, **** denotes p < 0.0001, ns denotes not significant