Fig. 4

Effects of APOE genotype and sex on numbers of microglial processes. a Representative image of a thresholded confocal stack labeled with ThioS (cyan outline) and Iba1 immunoreactive microglia (black) in the HPC. The image below shows an overlay with Sholl analysis grid. b Microglial process number within 20 μm of ThioS-labeled plaques in male (M) and female (F) EFAD mice with APOE3 (open bars) and APOE4 (filled bars) genotypes. c Microglial process number/cell within 20 μm of ThioS-labeled plaques. d Quantification of microglial process intersections at increasing distances from ThioS-labeled plaques in male (black lines) and female (red lines) EFAD mice with APOE3 (solid lines) and APOE4 (dashed lines) genotypes. Numbers of mice analyzed per group are as follows: male E3FAD (n = 6), female E3FAD (n = 5), male E4FAD (n = 4), and female E4FAD (n = 5). ns denotes not significant, * denotes p < 0.05, *** denotes p < 0.001