Fig. 1

Microglial plaque coverage in EFAD mice is diminished by APOE4 and female sex. a Representative images show ThioS-positive plaques (red) and Iba1-immunoreactive microglia (green) in the HPC of EFAD mice at 6 months of age. 3D images show composite, stacked images across the z-axis, whereas 2D images show individual z-sections with corresponding labeling across x- and y-axes. Scale bars = 5 μm. b Quantification of Iba1-immunoreactive processes in contact with ThioS-positive amyloid deposits (% plaque coverage) in male (M) and female (F) EFAD mice with APOE3 (open bars) and APOE4 (filled bars) genotypes. c-d Correlation plots of plaque perimeter in relation to microglial plaque coverage in APOE3 (c) and APOE4 (d) male and female mice. Numbers of mice analyzed per group are as follows: male E3FAD (n = 6), female E3FAD (n = 5), male E4FAD (n = 5), and female E4FAD (n = 6). * denotes p < 0.05, ** denotes p < 0.01 *** denotes p < 0.001