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Table 1 Cocktails of antibodies used for double and triple labeling immunohistochemistry

From: Endogenous mouse huntingtin is highly abundant in cranial nerve nuclei, co-aggregates to Abeta plaques and is induced in reactive astrocytes in a transgenic mouse model of Alzheimer’s disease

Primary antibody/stain Dilution Host Company Secondary antibody
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy2
ChAT 1:100 goat Millipore donkey anti-goat Cy3
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy2
DARPP-32 1:200 guinea pig Syn. Systems donkey anti-guinea pig Cy3
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy2
Ucn-1 1:100 goat Santa Cruz donkey anti-goat Cy3
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy2
TH 1:50 goat Santa Cruz donkey anti-goat Cy3
HTT (EP867Y) 1:1000 rabbit Abcam biotin-donkey anti-rabbit + SA-HRP
ThS 1% - Sigma -
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy3
Abeta (4G8) 1:2000 mouse Biolegend donkey anti-mouse Cy2
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy3
Iba1 1:200 guinea pig Syn. Systems donkey anti-mouse Cy5
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy3
GFAP (G-A-5) 1:1000 mouse Sigma donkey anti-mouse Cy5
HTT (EP867Y) 1:1000 rabbit Abcam donkey anti-rabbit Cy3
ThS 1% - Sigma -
GFAP (G-A-5) 1:1000 mouse Sigma donkey anti-mouse Cy5
  1. Secondary antibodies were all from Dianova and used at a dilution of 1:400
  2. HTT huntingtin, ChAT choline acetyltransferase, DARPP-32 dopamine and cAMP regulated phosphoprotein 32 kDa, Ucn-1 Urocortin-1, TH tyrosine hydroxylase, SA-HRP horseradish peroxidase-conjugated streptavidin, Iba1 ionized calcium-binding adapter molecule 1, ThS ThioflavinS, GFAP glial fibrillary acidic protein