Table 1 Cocktails of antibodies used for double and triple labeling immunohistochemistry
Primary antibody/stain | Dilution | Host | Company | Secondary antibody |
|---|---|---|---|---|
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
ChAT | 1:100 | goat | Millipore | donkey anti-goat Cy3 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
DARPP-32 | 1:200 | guinea pig | Syn. Systems | donkey anti-guinea pig Cy3 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
Ucn-1 | 1:100 | goat | Santa Cruz | donkey anti-goat Cy3 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy2 |
TH | 1:50 | goat | Santa Cruz | donkey anti-goat Cy3 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | biotin-donkey anti-rabbit + SA-HRP |
ThS | 1% | - | Sigma | - |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
Abeta (4G8) | 1:2000 | mouse | Biolegend | donkey anti-mouse Cy2 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
Iba1 | 1:200 | guinea pig | Syn. Systems | donkey anti-mouse Cy5 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
GFAP (G-A-5) | 1:1000 | mouse | Sigma | donkey anti-mouse Cy5 |
HTT (EP867Y) | 1:1000 | rabbit | Abcam | donkey anti-rabbit Cy3 |
ThS | 1% | - | Sigma | - |
GFAP (G-A-5) | 1:1000 | mouse | Sigma | donkey anti-mouse Cy5 |