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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Endogenous mouse huntingtin is highly abundant in cranial nerve nuclei, co-aggregates to Abeta plaques and is induced in reactive astrocytes in a transgenic mouse model of Alzheimer’s disease

Fig. 6

RT-qPCR and immunocytochemistry for HTT expression in mouse primary neuronal and astrocytic cultures. a In the upper lane the RT-qPCR products obtained with Htt primer pair #1 at the calculated product size of 152 bp are shown for primary neurons and astrocytes derived from three wild type (wt) and three Tg2576 (tg) mice. Note the similar expression levels for Htt mRNA in wt and tg neuronal and astrocytic cultures. The amount of Htt-specific PCR product was normalized to the expression of the housekeeping gene Cyclophilin A (CycA) (lower lane). b Immunocytochemistry reveals the presence of HTT protein (green fluorescence) in primary neurons (left) and primary astrocytes (right) as indicated by arrows. Neurons were identified by NeuN, HuC/D labelling (red fluorescence) and astrocytes were marked by GFAP immunocytochemistry (red fluorescence)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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