Table 1 Antibodies used for immunohistochemistry and multiple-antigen immunofluorescence staining in this study
Antibody | Host | Clonality | Immunogen | Company | Catalogue number | Dilution | Pretreatment | Secondary antibody |
|---|---|---|---|---|---|---|---|---|
Choline-acetyltransferase (ChAT) | Goat | Polyclonal | Human placental ChAT | Millipore | AB144P | 1:100 (IHC); 1: 50 (IF) | Pressure cooker, 0.01 M sodium citrate buffer (pH 6) | Alexa-Fluor® 488-conjugated donkey anti-goat antibody |
Tau (AT8; Phospho-PHF-tau pSer202 + Thr205) | Mouse | Monoclonal (IgG1) | Partially purified human PHF-Tau | Pierce Thermo Scientific | MN1020 | 1:100 (IF) | Nil | Alexa-Fluor® 568-conjugated or Alexa-Fluor® 647-conjugated donkey anti-mouse antibody |
Alpha-synuclein (Clone 42) | Mouse | Monoclonal (IgG1)/ Clone 42 | Rat Synuclein-1 aa. 15–123 | BD Transduction Laboratories | 610,787 | 1:100 (IF) | 10 mins 80% formic acid | Alexa-Fluor® 647-conjugated donkey anti-mouse antibody |
Tau (ps396; phosph-tau pSer396) | Rabbit | Polyclonal | Human Tau, serine 396 | Thermo Fisher Scientific | 44752G | 1:200 (IF) | Nil | Alexa-Fluor® 568-conjugated donkey anti-rabbit antibody |