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Table 1 Antibodies used for immunohistochemistry and multiple-antigen immunofluorescence staining in this study

From: Hippocampal CA2 Lewy pathology is associated with cholinergic degeneration in Parkinson’s disease with cognitive decline

Antibody Host Clonality Immunogen Company Catalogue number Dilution Pretreatment Secondary antibody
Choline-acetyltransferase (ChAT) Goat Polyclonal Human placental ChAT Millipore AB144P 1:100 (IHC); 1: 50 (IF) Pressure cooker, 0.01 M sodium citrate buffer (pH 6) Alexa-Fluor® 488-conjugated donkey anti-goat antibody
Tau (AT8; Phospho-PHF-tau pSer202 + Thr205) Mouse Monoclonal (IgG1) Partially purified human PHF-Tau Pierce Thermo Scientific MN1020 1:100 (IF) Nil Alexa-Fluor® 568-conjugated or Alexa-Fluor® 647-conjugated donkey anti-mouse antibody
Alpha-synuclein (Clone 42) Mouse Monoclonal (IgG1)/ Clone 42 Rat Synuclein-1 aa. 15–123 BD Transduction Laboratories 610,787 1:100 (IF) 10 mins 80% formic acid Alexa-Fluor® 647-conjugated donkey anti-mouse antibody
Tau (ps396; phosph-tau pSer396) Rabbit Polyclonal Human Tau, serine 396 Thermo Fisher Scientific 44752G 1:200 (IF) Nil Alexa-Fluor® 568-conjugated donkey anti-rabbit antibody
  1. Abbreviations: IF, immunofluorescence; IHC, immunohistochemistry