Choline-acetyltransferase (ChAT)
|
Goat
|
Polyclonal
|
Human placental ChAT
|
Millipore
|
AB144P
|
1:100 (IHC); 1: 50 (IF)
|
Pressure cooker, 0.01 M sodium citrate buffer (pH 6)
|
Alexa-Fluor® 488-conjugated donkey anti-goat antibody
|
Tau (AT8; Phospho-PHF-tau pSer202 + Thr205)
|
Mouse
|
Monoclonal (IgG1)
|
Partially purified human PHF-Tau
|
Pierce Thermo Scientific
|
MN1020
|
1:100 (IF)
|
Nil
|
Alexa-Fluor® 568-conjugated or Alexa-Fluor® 647-conjugated donkey anti-mouse antibody
|
Alpha-synuclein (Clone 42)
|
Mouse
|
Monoclonal (IgG1)/ Clone 42
|
Rat Synuclein-1 aa. 15–123
|
BD Transduction Laboratories
|
610,787
|
1:100 (IF)
|
10 mins 80% formic acid
|
Alexa-Fluor® 647-conjugated donkey anti-mouse antibody
|
Tau (ps396; phosph-tau pSer396)
|
Rabbit
|
Polyclonal
|
Human Tau, serine 396
|
Thermo Fisher Scientific
|
44752G
|
1:200 (IF)
|
Nil
|
Alexa-Fluor® 568-conjugated donkey anti-rabbit antibody
|