Fig. 6From: Hippocampal CA2 Lewy pathology is associated with cholinergic degeneration in Parkinson’s disease with cognitive declineDouble immunofluorescence staining between ChAT and αSN or tau. Post-acquisition image processing was performed to enhance the contrast of the images. On the top, double immunofluorescence staining with ChAT (green) and αSN (red) showed near complete co-localisation (yellow). Some ChAT-positive neuronal fibres had sparing of αSN pathology (white arrows). At the bottom, double immunofluorescence staining with ChAT (green) and AT8 tau (red) only had minimal colocalisation (white arrowheads). Insets showing co-localisation in higher magnification. Scale bars = 20 μmBack to article page