Fig. 3From: Hippocampal CA2 Lewy pathology is associated with cholinergic degeneration in Parkinson’s disease with cognitive declineQuantification of cholinergic varicosities in the CA2 hippocampal subfield. Acquired imaged from the confocal microscope (a) was converted to monochrome by extraction of the green channel (b). The image was then colour-inverted (c) and subsequently, the percentage area coverage of ChAT was obtained with manual thresholding on the ImagePro Plus software. Scale bar = 20 μmBack to article page