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Fig. 6 | Acta Neuropathologica Communications

Fig. 6

From: Parkinson’s disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes

Fig. 6

Astrocytes with ER stress-induced abnormalities affect neuronal survival. a Neurons isolated from non-Tg or LRRK2-GS mice were layered on top of non-Tg or LRRK2-GS astrocytes for 7–10 days in vitro, allowing contact between neurons and astrocytes. Representative double-immunofluorescence staining of direct co-cultures with anti-GFAP and Tuj1 antibodies; cells were incubated in the absence or presence of α-synuclein (α-Syn). b, c Summary data showing the fluorescence intensities of Tuj1 per cell (n = 25) (b) and average length of neurites per cell (n = 30 cells) (c). d-f Organotypic slices from non-Tg and LRRK2-GS mice were treated with α-Syn for 72 h. Representative images of TUNEL staining (d), and graphs showing fluorescence intensities of MAP2 per each independent area (n = 5) (e) and TUNEL-positive cells per mm2 (n = 6) (f). All data are presented as means ± SD of three independent experiments (*p < 0.05)

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