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Fig. 1 | Acta Neuropathologica Communications

Fig. 1

From: Parkinson’s disease-associated LRRK2-G2019S mutant acts through regulation of SERCA activity to control ER stress in astrocytes

Fig. 1

The LRRK2-GS mutant induces ER stress and apoptosis in astrocytes. a, b Astrocytes isolated from non-Tg (nTg) and LRRK2-GS (GS) mice were treated with monomeric (mono) or oligomeric (oligo) α-synuclein (α-Syn) for 48 h. Expression levels of the indicated mRNAs (a) and proteins (b) were analyzed by real-time qPCR and Western blotting, respectively. Crosshatching denotes a cleaved caspase-12 band. c Representative images (upper) and summary data (lower) showing TUNEL staining in astrocytes from non-Tg and LRRK2-GS mice treated with α-Syn for 72 h. d, e Organotypic slices from non-Tg and GS mice were treated with α-Syn (1 μM) for 48 h (d) or 72 h (e). Western blot showing expression levels of the indicated proteins (d) and representative images (left) and summary data (right) showing CHOP and TUNEL staining (e). All data are presented as means ± SD of three independent experiments (*p < 0.05)

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Acta Neuropathologica Communications

ISSN: 2051-5960

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