Fig. 4
Administration of Delta-24-RGD in combination with radiotherapy heightens the immune infiltration in DIPG murine tumors. a Representative images of immune infiltration, such as perivascular cuffing, after Delta-24-RGD/RT treatment. The mouse brain stained against CD3 (a), CD4 (b) and CD8 (c). d Quantification of positive CD3+, e CD4+, and f CD8+ cell infiltration per mm2 of DIPG tumors. Graph showing the quantification of positive cells infiltrating the brain 15 days after the indicated treatments per mm2 (PBS, RT, Delta-24-RGD and Delta-24-RGD/RT; n = 2–3). Comparisons were analyzed with One-way ANOVA. g Quantification of Granzyme B, IFN gamma, CD8a and CD4 mRNA expression. The data shown represent mRNA expression in tumors treated with Delta-24-RGD, RT, and Delta-24-RGD/RT normalized to the average of PBS-tumors mRNA expression (n = 2). Data were analyzed with One-way ANOVA