Fig. 3

Combination of Delta-24-RGD/radiotherapy exerts a potent oncolytic effect in the NP53 and XFM murine DIPG cell lines. a Analyses of the expression of viral late protein fiber in murine cell lines 42 h after the indicated treatments by western blotting. b Quantification of Delta-24-RGD replication in the indicated cell lines. Viral titers were determined 3 days after infection with Delta-24-RGD (100 MOIs) and irradiation with either 3, 6 or 12 Gy. The viral titers were quantified using the anti-hexon staining-based method in 293 cells and expressed as plaque-forming units (pfu) per milliliter. The data are shown as the mean ± SD of three independent experiments. c Cell viability analyses of the combination treatment in DIPG murine cell lines. Cell viability was assessed 5 days after irradiation and/or viral infection using an automatic cell counter that measures cell viability (life, death and total cells) with the standard trypan blue reaction. Data are shown as the percentage (mean ± SD) of viability after irradiation at the indicated doses alone or also infected with Delta-24-RGD and analyzed with Two-tailed Student t-test