Fig. 2

Delta-24-RGD downregulation of the cellular DNA damage repair machinery in the DIPG and pHGG cell lines. a Expression analyses by western blotting of the relevant proteins involved in the DNA damage response to RT in the DIPG and pHGG cell lines after the indicated treatments. The proteins levels were evaluated 72 h after cells were treated. b Evaluation of DNA damage upon treatment with Delta-24-RGD and/or RT by the comet assay. TP54 cells were administered the indicated treatments, and 72 h later, the induction of comets was assessed. Representative photomicrographs of comets shown by the cells after the indicated treatment (magnification, × 200). c Quantification of positive cells showing comets after the indicated treatment. Data are shown as the percentage of comet tails found per treatment percentage (n = 500 cells per treatment); bars represent means ± SD. All experiments were performed in triplicate and analyzed using two-way ANOVA and corrected for multiple comparision with Bonferroni posttest; **, P < 0.01 and ***, P < 0.001. d Hexon immune-staining representative images (scale bar =100um) after the indicated treatments. The above images images show differences in hexon protein expression in CHLA-03-AA tumors while below images show hexon staining in TP54 tumors