Skip to main content
Fig. 5 | Acta Neuropathologica Communications

Fig. 5

From: eIF4B and eIF4H mediate GR production from expanded G4C2 in a Drosophila model for C9orf72-associated ALS

Fig. 5

eIF4B and eIF4H1 RNAi selectively reduce GR-GFP levels produced from LDS-(G4C2)EXP. a. Fluorescence imaging in LDS-(G4C2)EXP expressing flies shows that depletion of eIF4B or eIF4H by RNAi results in reduced GR-GFP levels (GMR-GAL4). b. Blinded quantification of GR-GFP signal in LDS-(G4C2)EXP animals relative to the signal in control RNAi animals. Analysis of GR-GFP puncta number and size are shown in black. Total GR-GFP signal is shown in grey. n = 6–7 animals per genotype. Further, qPCR was used to quantify RNA levels in LDS-(G4C2)EXP flies co-expressing control, eIF4B, or eIF4H1 RNAi, shown in light grey. c. A control fluorescent protein, DsRed, was similarly expressed in the fly eye with RNAi to control, eIF4B, or eIF4H. d. Blinded quantification of DsRed fluorescence shows no effect by RNAi. n = 6 animals per genotype. e. A representative western immunoblot image for β-Galactosidase in LacZ flies co-expressing control, eIF4B, or eIF4H1 RNAi. Uncropped westerns (Additional file 1: Figure S4). Blinded quantification of β-Galactosidase western immunoblots normalized to the loading control, Tubulin. For graphs, shown are individual data points from 2 independent assays with mean ± SEM. Statistics: one-way ANOVAs with Tukey’s multiple comparison correction, p-values **** < 0.0001, *** < 0.001, ** < 0.01, * < 0.05, no significance > 0.05. RNAi lines: control (JF01355), eIF4B (HMS04503), eIF4H1 (HMS04504). For full genotypes see Additional file 7: Table S1

Back to article page