Fig. 3

Analysis of eIF4B and eIF4H1 RNAi in control flies. a. RNA levels produced from eIF4B or eIF4H1 were assessed by qPCR in flies ubiquitously expressing RNAi (by Daughterless-GAL4). Statistics: one-way ANOVAs with Tukey’s multiple comparison correction, p-values **** < 0.0001, *** < 0.001, ** < 0.01, * < 0.05, no significance > 0.05. Shown: individual data points from 2 independent experiments with mean ± SEM. b. Viability studies in Drosophila reveal that RNAi-depletion of eIF4B or eIF4H1 does not significantly alter the number of adult flies expected to eclose. Shown: ratio of progeny from two individual crosses with RNAi compared to sibling animals with the balancer chromosome that reach adulthood (1-2d adult animals). Comparing the # progeny that eclose from a single vial compensates for differences in mating variability, fertilized eggs laid, among other variables. However, we note that the presence of the balancer chromosome could potentially cause mild sub-viability to adulthood. Crosses: RNAi/CyO x Da-GAL4 (III); counted progeny: RNAi/+; Da-GAL4/+ and CyO/+; Da-GAL4/+. RNAi lines: control (JF01355), eIF4B RNAi (HMS04503), eIF4H1 RNAi (HMS04504). For full genotypes see Additional file 7: Table S1