Fig. 5

ALYREF protein levels are increased in human ALS patient motor neurons. a Schematic depicting the location of spinal cord motor neurons imaged in (b). b Fluorescent immunostaining of ALYREF in lumbar spinal cord sections from control and ALS cases. 2 independent ALYREF antibodies were used. Scale bar 50 μm. c Image intensity quantification using the rabbit polyclonal antibody targeting ALYREF protein. Control n = 83 cells from n = 9 cases. C9- ALS n = 27 cells from n = 3 cases. C9+ ALS n = 38 cells from n = 3 cases. ****p < 0.0001, **p < 0.01, *p < 0.05 One-way ANOVA, Tukey’s multiple comparisons test, F(2,145) = 23.87. For all graphs, individual data points are shown with mean +/− standard deviation